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Can I read Use of Avidin-sepharose to Lsolate and Identify Biotin Polypeptides from Crude Extracts on EtoBox?
Use of Avidin-sepharose to Lsolate and Identify Biotin Polypeptides from Crude Extracts by Judith A. Swack; Gary L. Zander; Merton F. Utter is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Use of Avidin-sepharose to Lsolate and Identify Biotin Polypeptides from Crude Extracts about?
A method of isolating and identifying biotin polypeptides from crude cellular extracts is described. Protein samples are run on small avidin-Sepharose columns. After washing away nonspecifically bound protein, the biotin enzymes are eluted using an SDS-urea solution. The inactive polypeptides are then electrophoresed on SDS-polyacrylamide gels. The biotin polypeptides in the gels are identified by using fluorescent avidin or by analyzing for radioactive biotin-labeled polypeptides. A sensitive method for assaying biotin using avidin-Sepharose is also described.
Who reads Use of Avidin-sepharose to Lsolate and Identify Biotin Polypeptides from Crude Extracts?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Judith A. Swack; Gary L. Zander; Merton F. Utter
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 1978
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)