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Extracellular nucleotides regulate CCL20 release from human primary airway epithelial cells, monocytes and monocyte‐derived dendritic cells by Brice Marcet; Michael Horckmans; Frédérick Libert; Sergio Hassid; Jean‐Marie Boeynaems; Didier Communi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Extracellular nucleotides regulate ion transport and mucociliary clearance in human airway epithelial cells (HAECs) via the activation of P2 receptors, especially P2Y~2~. Therefore, P2Y~2~ receptor agonists represent potential pharmacotherapeutic agents to treat cystic fibrosis (CF). Nucleotides also modulate inflammatory properties of immune cells like dendritic cells (DCs), which play an important role in mucosal immunity. Using DNA‐microarray experiments, quantitative RT‐PCR and cytokine measurements, we show here that UTP up‐regulated ∼2‐ to 3‐fold the antimicrobial chemokine CCL20 expression and release in primary HAECs cultured on permeable supports at an air–liquid interface (ALI). Both P2Y~2~ (ATPγS, UTP, INS365) and P2Y~6~ (UDP, INS48823) agonists increased CCL20 release. UTP‐induced CCL20 release was insensitive to NF‐κB pathway inhibitors but sensitive to inhibitors of ERK1/2 and p38/MAPK pathways. Furthermore, UTP had no effect on interleukin‐(IL)‐8 release and reduced the release of both CCL20 and IL‐8 induced by TNF‐α and LPS. Accordingly, UTP reduced the capacity of basolateral supernatants of HAECs treated with TNF‐α or LPS to induce the chemoattraction

Who reads Extracellular nucleotides regulate CCL20 release from human primary airway epithelial cells, monocytes and monocyte‐derived dendritic cells?

Author

Brice Marcet; Michael Horckmans; Frédérick Libert; Sergio Hassid; Jean‐Marie Boeynaems; Didier Communi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)