Can I read Regulation of osteoblast and osteoclast functions by FGF-6 on EtoBox?

Regulation of osteoblast and osteoclast functions by FGF-6 by Michela Bosetti; Massimiliano Leigheb; Roger A. Brooks; Francesca Boccafoschi; Mario F. Cannas is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Regulation of osteoblast and osteoclast functions by FGF-6 about?

## Abstract Fibroblast growth factor‐6 (FGF‐6) is known to be the key ligand for fibroblast growth factor receptor 4 (FGFR4) during muscle regeneration but its role in bone has yet to be verified. FGFR signaling is known to be important in the initiation and regulation of osteogenesis, so in this study the actions of FGF‐6 on human osteoblasts and osteoclasts were investigated. Human primary osteoblasts (hOB) were used to study the effect of FGF‐6 on proliferation (by ATP quantification), signal transduction (by ERK and AKT phosphorylation), differentiation (by alkaline phosphatase activity, APA), and mineralization (by calcein staining). To study FGF‐6 activity on osteoclast differentiation, human bone marrow cells were used and tartrate‐resistant acid phosphatase (TRAP) multinucleated cells together with actin filaments arrangements were quantified. Human primary mature osteoclasts were used to evaluate the effect of FGF‐6 on osteoclast reabsorbing activity by reabsorbed pit measurements. FGF‐6 >10^−9^ M as FGF‐2 10^−7^ M induced hOB proliferation mediated by pERK together with a reduction in APA and reduced mineralization of the treated cells. Moreover FGF‐6 increased the format

Who reads Regulation of osteoblast and osteoclast functions by FGF-6?

Author

Michela Bosetti; Massimiliano Leigheb; Roger A. Brooks; Francesca Boccafoschi; Mario F. Cannas

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)