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Tissue specific metabolism of 1α,25‐dihydroxy‐20‐epi‐vitamin D~3~ into new metabolites with significant biological activity: Studies in rat osteosarcoma cells (UMR 106 and ROS 17/2.8) by M-L. Siu-Caldera; D. Sunita Rao; N. Astecker; A. Weiskopf; P. Vouros; K. Konno; T. Fujishima; H. Takayama; S. Peleg; G. Satyanarayana Reddy is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract In a recent study, we investigated the metabolism of 1α,25‐dihydroxy‐20‐epi‐vitamin D~3~ (1α,25(OH)~2~‐20‐epi‐D~3~), a potent synthetic vitamin D~3~ analog in the isolated perfused rat kidney and proposed that the enhanced biological activity of 1α,25(OH)~2~‐20‐epi‐D~3~ is in part due to its metabolism into stable bioactive intermediary metabolites derived via the C‐24 oxidation pathway (Siu‐Caldera et al. [1999] J. Steroid. Biochem. Mol. Biol. 71:111–121). It is now well established that 1α,25(OH)~2~D~3~ and its analogs are metabolized in target tissues not only via the C‐24 oxidation pathway but also via the C‐3 epimerization pathway. As the perfused rat kidney does not express the C‐3 epimerization pathway, we could not identify other possible bioactive metabolites of 1α,25(OH)~2~‐20‐epi‐D~3~ such as 1α,25(OH)~2~‐20‐epi‐3‐epi‐D~3~, derived via the C‐3 epimerization pathway. Therefore, we studied the metabolism of 1α,25(OH)~2~‐20‐epi‐D~3~ in rat osteosarcoma cells (UMR 106) which express both the C‐24 oxidation and the C‐3 epimerization pathways. Our results indicate that 1α,25(OH)~2~‐20‐epi‐D~3~ is metabolized in UMR 106 cells into several metabolites which included

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Author

M-L. Siu-Caldera; D. Sunita Rao; N. Astecker; A. Weiskopf; P. Vouros; K. Konno; T. Fujishima; H. Takayama; S. Peleg; G. Satyanarayana Reddy

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)