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A High-resolution Colorimetric Immunoassay for Tyramine Detection Based on Enzyme-enabled Growth of Gold Nanostar Coupled with Smartphone Readout by Lin Luo; Shuang-Zi Luo; Bao-Zhu Jia; Wen-Feng Zhang; Hong Wang; Xiao-Qun Wei; Yu-Dong Shen; Hong-Tao Lei; Zhen-Lin Xu; Jin-Yi Yang is a Agricultural and Biological Sciences article available to read on EtoBox.
What is A High-resolution Colorimetric Immunoassay for Tyramine Detection Based on Enzyme-enabled Growth of Gold Nanostar Coupled with Smartphone Readout about?
In this work, a specific monoclonal antibody against tyramine was produced based on a new hapten design. Then, we developed a high-resolution multicolor colorimetric immunoassay for tyramine based on this antibody by integrating enzyme-induced multicolor generation with smartphone-assistant signal readout. The multicolor generation is due to the shift of the local surface plasmon resonance band of gold nanostructure controlled by alkaline phosphatase-induced the growth of gold nanostars. Quantitative detection of tyramine was achieved via analyzing the red/blue channel values of assay solution's image taken by a smartphone with the support of a color recognizer application. The limit of detection of this immunoassay for tyramine detection in beef, pork and yoghurt was 19.7 mg/kg or L. The average recoveries were between 83 % and 103 %., and the results were validated by high performance liquid chromatography to be reliable. Overall, this developed immunoassay provides a promising platform for on-site detection of tyramine.
Who reads A High-resolution Colorimetric Immunoassay for Tyramine Detection Based on Enzyme-enabled Growth of Gold Nanostar Coupled with Smartphone Readout?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Lin Luo; Shuang-Zi Luo; Bao-Zhu Jia; Wen-Feng Zhang; Hong Wang; Xiao-Qun Wei; Yu-Dong Shen; Hong-Tao Lei; Zhen-Lin Xu; Jin-Yi Yang
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)