Can I read Identification of Hyaluronic Acid Oligosaccharides by Direct Coupling of Capillary Electrophoresis with Electrospray Ion Trap Mass Spectrometry on EtoBox?

Identification of Hyaluronic Acid Oligosaccharides by Direct Coupling of Capillary Electrophoresis with Electrospray Ion Trap Mass Spectrometry by Andrea V. Kühn; Hans H. Rüttinger; Reinhard H. H. Neubert; Klaus Raith is a Chemistry article available to read on EtoBox.

What is Identification of Hyaluronic Acid Oligosaccharides by Direct Coupling of Capillary Electrophoresis with Electrospray Ion Trap Mass Spectrometry about?

## Abstract A new method for the identification of oligosaccharides obtained by enzymatic digestion of hyaluronic acid (HA) with bacterial hyaluronidase (HA lyase, E.C. 4.2.2.1, from __Streptococcus agalactiae__) using online capillary electrophoresis/electrospray mass spectrometry (CE/ESI‐MS) is presented. A fused‐silica capillary coated with polyacrylamide was used with a 40 mM ammonium acetate buffer at pH 9.0 and a separation voltage of +30 kV applied to the inlet. Separation was achieved for oligosaccharides containing 4–16 monomers. The migration behavior follows the chain length of the oligomers, regardless of charge state. However, no linear relationship was found for the relation between mobility and chain length. Using an ion trap mass analyzer, complementary structural information was obtained by MS/MS and MS^n^ experiments. Copyright © 2003 John Wiley & Sons, Ltd.

Who reads Identification of Hyaluronic Acid Oligosaccharides by Direct Coupling of Capillary Electrophoresis with Electrospray Ion Trap Mass Spectrometry?

Author

Andrea V. Kühn; Hans H. Rüttinger; Reinhard H. H. Neubert; Klaus Raith

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2003

Language

EN

Field

Chemistry (Physical Sciences)