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Crosstalk of Osteoblast and Osteoclast Precursors on Mineralized Collagen—towards an in Vitro Model for Bone Remodeling by A. Bernhardt; S. Thieme; H. Domaschke; A. Springer; A. Rösen-Wolff; M. Gelinsky is a Engineering article available to read on EtoBox.

What is Crosstalk of Osteoblast and Osteoclast Precursors on Mineralized Collagen—towards an in Vitro Model for Bone Remodeling about?

## Abstract Bone remodeling and, therefore, integration of implant materials require the coordinated regulation of osteoblast and osteoclast activity. This is why the __in vitro__ evaluation of biomaterials for bone regeneration should involve not only the analysis of osteoblast differentiation but also the formation and differentiation of osteoclasts. In the present study, we applied a material made of mineralized collagen I that mimics extracellular bone matrix to establish a culture system, which allows the cocultivation of human monocytes and human mesenchymal stem cells (hMSCs), which were differentiated into osteoclast‐like cells and osteoblasts, respectively. Both cell types were cultivated on membrane‐like structures from mineralized collagen. Transwell inserts were used to spatially separate the cell types but allowed exchange of soluble factors. The osteoclastogenesis and osteogenic differentiation were evaluated by analysis of gene expression, determination of alkaline phosphatase (ALP), and tartrate‐resistant acidic phosphatase (TRAP) activity. Furthermore, cell morphology was studied using scanning electron and transmission electron microscopy. Osteogenically induced h

Who reads Crosstalk of Osteoblast and Osteoclast Precursors on Mineralized Collagen—towards an in Vitro Model for Bone Remodeling?

Author

A. Bernhardt; S. Thieme; H. Domaschke; A. Springer; A. Rösen-Wolff; M. Gelinsky

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)

Published

2010

Language

EN

Field

Engineering (Physical Sciences)