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Can I read Basement Membrane Ligands Initiate Distinct Signalling Networks to Direct Cell Shape on EtoBox?
Basement Membrane Ligands Initiate Distinct Signalling Networks to Direct Cell Shape by Randles, Michael J.; Lausecker, Franziska; Humphries, Jonathan D.; Byron, Adam; Clark, Simon J.; Miner, Jeffrey H.; Zent, Roy; Humphries, Martin J.; Lennon, Rachel is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Basement Membrane Ligands Initiate Distinct Signalling Networks to Direct Cell Shape about?
Cells have evolved mechanisms to sense the composition of their adhesive microenvironment. Although much is known about general mechanisms employed by adhesion receptors to relay signals between the extracellular environment and the cytoskeleton, the nuances of ligand-specific signalling remain undefined. Here, we investigated how glomerular podocytes, and four other basement membrane-associated cell types, respond morphologically to different basement membrane ligands. We defined the composition of the respective adhesion complexes using mass spectrometry-based proteomics. On type IV collagen, all epithelial cell types adopted a round morphology, with a single lamellipodium and large adhesion complexes rich in actin-binding proteins. On laminin (511 or 521), all cell types attached to a similar degree but were polygonal in shape with small adhesion complexes enriched in endocytic and microtubule-binding proteins. Consistent with their distinctive morphologies, cells on type IV collagen exhibited high Rac1 activity, while those on laminin had elevated PKCα. Perturbation of PKCα was able to interchange morphology consistent with a key role for this pathway in matrix ligand-specific
Who reads Basement Membrane Ligands Initiate Distinct Signalling Networks to Direct Cell Shape?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Randles, Michael J.; Lausecker, Franziska; Humphries, Jonathan D.; Byron, Adam; Clark, Simon J.; Miner, Jeffrey H.; Zent, Roy; Humphries, Martin J.; Lennon, Rachel
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0945-053X)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)