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Multiple sclerosis: Serum-derived exosomes express myelin proteins by Grazyna Galazka; Marcin P Mycko; Igor Selmaj; Cedric S Raine; Krzysztof W Selmaj is a Medicine article available to read on EtoBox.

What is Multiple sclerosis: Serum-derived exosomes express myelin proteins about?

## Background: Exosomes are small extracellular vesicles that provide cell-to-cell communication and are involved in immunoregulation. ## Objective: To investigate serum exosomes for the presence of myelin proteins outside the central nervous system (CNS) and their role in multiple sclerosis (MS). ## Methods: Serum, cerebrospinal fluid (CSF), and peripheral blood mononuclear cell (PBMC) samples were collected from 45 patients with relapsing–remitting MS (RRMS), 30 patients with secondary progressive MS (SPMS), and 45 healthy controls. Exosomes were isolated using a polymer formulation method, and their size, concentration, and CNS myelin protein contents were measured by a nanoparticle tracking analysis, enzyme-linked immunosorbent assays, and Western blot. ## Results: We found that exosomes expressed three major myelin proteins, myelin basic protein, proteolipid protein, and myelin oligodendrocyte glycoprotein (MOG). Exosomal content of MOG strongly correlated with disease activity and was highest in RRMS patients in relapse and in SPMS patients. Serum-derived exosomes induced proliferation of MOG-T cell receptor transgenic T cells confirming that serum exosomes maintained MOG imm

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Author

Grazyna Galazka; Marcin P Mycko; Igor Selmaj; Cedric S Raine; Krzysztof W Selmaj

Publisher

SAGE Publications

Published

2017

Language

EN

Field

Medicine (Health Sciences)