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Mitogen Activated Protein Kinase-dependent Inhibition of Osteocalcin Gene Expression by Transforming Growth Factor-β1 by Sukyee Kwok; Nicola C. Partridge; Narasaimhan Srinivasan; Shantikumar V. Nair; Nagarajan Selvamurugan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract TGF‐β (transforming growth factor‐beta) plays a key role in osteoblast differentiation and bone development. While the ability of TGF‐β to inhibit the expression of osteoblast differentiation genes has been well documented, the mechanism of this inhibition is not yet completely characterized. Runx2, a transcription factor necessary for expression of osteoblast differentiation genes is a central target of inhibition by TGF‐β. In this study, we found that TGF‐β1 inhibits expression of osteoblast differentiation genes without altering expression of Runx2. Transient transfection experiments determined that TGF‐β1 inhibited osteocalcin promoter activity and this effect is mediated through Runx2. We further identified that there was no change in protein expression, cellular localization, or DNA binding affinity of Runx2 after TGF‐β1‐treatment of osteoblasts, suggesting that Runx2 undergoes post‐translational modifications following TGF‐β1 treatment. Co‐immunoprecipitation experiments identified increased phosphorylation of Runx2 when differentiating osteoblasts were treated with TGF‐β1. Mitogen activated protein kinase (MAPK) inhibitors relieved the TGF‐β1‐inhibitory effect o

Who reads Mitogen Activated Protein Kinase-dependent Inhibition of Osteocalcin Gene Expression by Transforming Growth Factor-β1?

Author

Sukyee Kwok; Nicola C. Partridge; Narasaimhan Srinivasan; Shantikumar V. Nair; Nagarajan Selvamurugan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)