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Whole mitochondrial genomes assembled from thermally altered forensic bones and teeth 1 1 Introduction 1 2 Materials and methods 2 2.1 Sampling and burn category classification 2 2.2 DNA extraction 2 2.2.1 Loreille and colleagues extraction protocol 2 2.2.2 Modified Dabney and colleagues extraction protocol 2 2.2.3 Library preparation and indexing PCR 3 2.2.4 Mitochondrial DNA enrichment 3 2.2.5 Sequencing 3 3 mtDNA genome assembly and analysis 3 3.1 MitoPipe 1.0: Mitochondrial genome assembly and haplogroup assignment 3 3.2 Contamination control and DNA authentication 4 4 Results and discussion 4 4.1 Contamination and sample omission 4 4.2 Extraction type 4 4.3 Burn category mtDNA sequence variation 5 4.4 Within burn category mtDNA sequence variation by extraction type 5 4.5 mtDNA haplogroup composition 7 4.6 EMPOP database sequence queries 8 4.7 mapDamage2.0 results: burn category IV and V libraries 9 5 Conclusions 10 CRediT authorship contribution statement 10 Conflict of interest 10 Acknowledgements 10 Appendix A Supporting information 10 References 11

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EN

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nonfiction