Can I read Enzyme Assays: A Practical Approach (The ^APractical Approach Series) on EtoBox?

Enzyme Assays: A Practical Approach (The ^APractical Approach Series) by Robert Eisenthal and Michael J. Danson is a book available to read on EtoBox.

What is Enzyme Assays: A Practical Approach (The ^APractical Approach Series) about?

Enzyme assays are one of the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific examples. Chapters contain experimental protocols, including photomeric, electrochemical, radiochemical, and HPLC techniques, along with methods for determining enzyme activity after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also discussed are the techniques and problems associated with enzyme extraction, statistical analysis of kinetic data, and a chapter examining buffers and methods of protein estimation. This work will be valuable not only to biochemists, but to researchers in all areas of the life sciences.

Author

Robert Eisenthal and Michael J. Danson

Publisher

Oxford [England] ; New York: IRL Press at Oxford University Press

Published

1992

Language

EN

ISBN

9780199631438

Subjects

Science, Medical, Biology

Updated

2026-03-24