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Preparation of low-affinity red cells with dimethylsulfoxide-mediated inositol hexaphosphate incorporation: Hemoglobin and ATP recovery using a continuous-flow method by Dr. R. S. Franco; K. Wagner; M. Weiner; O. J. Martelo is a Medicine article available to read on EtoBox.

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Incorporation of IHP into red cells decreases oxygen affinity as a result of the binding of this compound to the 2,3-DPG site of hemoglobin. This investigation describes a continuous-flow method which utilizes the osmotic pulse technique to transport I H P into RBC. Using this procedure, it is possible to obtain a significant increase in P50 while maintaining in vitro cellular integrity. For example, IHP incorporation sufficient to cause an increase in the PSo of 20 mm Hg may be achieved with recovery of approximately 75 % of the hemoglobin and with maintenance of ATP levels compatible with good viability. The continuous-flow method allows uniform treatment of large, unit-size volumes of red cells with a relatively small quantity of reagents. The final cell product is macrocytic/hypochromic with an increased number of stomatocytes.

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Author

Dr. R. S. Franco; K. Wagner; M. Weiner; O. J. Martelo

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0361-8609)

Published

1984

Language

EN

Field

Medicine (Health Sciences)