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Purification and characterization of an inducible dissimilatory type sulfite reductase from Clostridium pasteurianum by Gail Harrison; Carol Curle; Edward J. Laishley is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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An inducible sulfite reductase was purified from Clostridium pasteurianum. The pH optimum of the enzyme is 7.5 in phosphate buffer. The molecular weight of the reductase was determined to be 83,600 from sodium dodecyl sulfate gel electrophoresis with a proposed molecular structure: o~2fl 2. Its absorption spectrum showed a maximum at 275 nm, a broad shoulder at 370 nm and a very small absorption maximum at 585 nm. No siroheme chromophore was isolated from this reductase. The enzyme could reduce the following substrates in preferential order: NH2OH > SeO~-> NO~-at rates 50% or less of its preferred substrate SO32-. The proposed dissimilatory intermediates, $30 2-or $20 2 , were not utilized by this reductase while KCN inhibited its activity. Varying the substrate concentration [SO 2-] from I to 2.5 gmol affected the stoichiometry of the enzyme reaction by alteration of the ratio of H2 uptake to S 2-formed from 2.5:1 to 3.1:1. The inducible sulfite reductase was found to be linked to ferredoxin which could be completely replaced by methyl viologen or partially by benzyl viologen. Some of the above-mentioned enzyme properties and physiological considerations indicated that it was a di

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Author

Gail Harrison; Carol Curle; Edward J. Laishley

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Archie Comics (ISSN 0302-8933)

Published

1984

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)