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Process optimization for mass production of 2,3-butanediol by Bacillus subtilis CS13 by Wang, Dexin ;Oh, Baek-Rock ;Lee, Sungbeom ;Kim, Dae-Hyuk ;Joe, Min-Ho is a Engineering article available to read on EtoBox.

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## Abstract ## Background Bacillus subtilis CS13 was previously isolated for 2,3-butanediol (2,3-BD) and poly-γ-glutamic acid (γ-PGA) co-production. When culturing this strain without L-glutamic acid in the medium, 2,3-BD is the main metabolic product. 2,3-BD is an important substance and fuel with applications in the chemical, food, and pharmaceutical industries. However, the yield and productivity for the B. subtilis strain should be improved for more efficient production of 2,3-BD. ## Results The medium composition, which contained 281.1 g/L sucrose, 21.9 g/L ammonium citrate, and 3.6 g/L MgSO~4~·7H~2~O, was optimized by response surface methodology for 2,3-BD production using B. subtilis CS13. The maximum amount of 2,3-BD (125.5 ± 3.1 g/L) was obtained from the optimized medium after 96 h. The highest concentration and productivity of 2,3-BD were achieved simultaneously at an agitation speed of 500 rpm and aeration rate of 2 L/min in the batch cultures. A total of 132.4 ± 4.4 g/L 2,3-BD was obtained with a productivity of 2.45 ± 0.08 g/L/h and yield of 0.45 g~2,3-BD~/g~sucrose~ by fed-batch fermentation. The meso-2,3-BD/2,3-BD ratio of the 2,3-BD produced by B. subtilis CS13 wa

Who reads Process optimization for mass production of 2,3-butanediol by Bacillus subtilis CS13?

Author

Wang, Dexin ;Oh, Baek-Rock ;Lee, Sungbeom ;Kim, Dae-Hyuk ;Joe, Min-Ho

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Engineering (Physical Sciences)