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Can I read Notch Signaling-modified Mesenchymal Stem Cells Improve Tissue Perfusion by Induction of Arteriogenesis in a Rat Hindlimb Ischemia Model on EtoBox?
Notch Signaling-modified Mesenchymal Stem Cells Improve Tissue Perfusion by Induction of Arteriogenesis in a Rat Hindlimb Ischemia Model by Shusaku Maeda; Shigeru Miyagawa; Takuji Kawamura; Takashi Shibuya; Kenichi Watanabe; Takaya Nakagawa; Akima Harada; Dai Chida; Yoshiki Sawa is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Notch Signaling-modified Mesenchymal Stem Cells Improve Tissue Perfusion by Induction of Arteriogenesis in a Rat Hindlimb Ischemia Model about?
## Abstract Notch signaling-modified human mesenchymal stem cell, SB623 cell, is a promising cell therapy product for ischemic stroke. With the aim to expand indications for their use for critical limb-threatening ischemia (CLTI), we hypothesized that SB623 cells improved tissue perfusion by inducing angiogenesis or arteriogenesis in a hindlimb ischemia model rat. In Sprague–Dawley rats, hindlimb ischemia was generated by femoral artery removal, then seven days after ischemic induction 1 × 10^5^ SB623 cells or PBS was injected into the ischemic adductor muscle. As compared with the PBS group, tissue perfusion was significantly increased in the SB623 group. While capillary density did not vary between the groups, αSMA- and vWF-positive arterioles with a diameter > 15 μm were significantly increased in the SB623 group. Whole transcriptome analysis of endothelial cells co-cultured with SB623 cells showed upregulation of the Notch signaling pathway as well as several other pathways potentially leading to arteriogenesis. Furthermore, rat muscle treated with SB623 cells showed a trend for higher ephrin-B2 and significantly higher EphB4 expression, which are known as arteriogenic markers
Who reads Notch Signaling-modified Mesenchymal Stem Cells Improve Tissue Perfusion by Induction of Arteriogenesis in a Rat Hindlimb Ischemia Model?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Shusaku Maeda; Shigeru Miyagawa; Takuji Kawamura; Takashi Shibuya; Kenichi Watanabe; Takaya Nakagawa; Akima Harada; Dai Chida; Yoshiki Sawa
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)