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This document discusses applying ribosome engineering technology to improve vitamin B12 production by Propionibacterium shermanii. Mutants resistant to rifampicin, gentamicin, and erythromycin were generated and showed a 5.2-fold higher per cell production of vitamin B12, though overall production did not change due to reduced cell growth. Real-time qPCR revealed the vitamin B12 biosynthesis genes were transcriptionally activated in the mutants, providing a mechanism for the higher yields.

Author

dian

Language

EN