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Detection and molecular identification of Leucocytozoon and Plasmodium species from village chickens in different areas of Myanmar by Shwe Yee Win; Hla Myet Chel; Myint Myint Hmoon; Lat Lat Htun; Saw Bawm; Mar Mar Win; Shiro Murata; Nariaki Nonaka; Ryo Nakao; Ken Katakura is a Medicine article available to read on EtoBox.

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Village chicken production, a traditional, small-scale, and extensive backyard poultry industry, has been profitable for local farmers in Myanmar. However, there is scanty information available concerning the infection of these chickens with avian pathogens, including haemoprotozoan parasites. In the present study, we provide the first report of microscopic detection and molecular identification of Leucocytozoon and Plasmodium parasites from seven different areas of Myanmar. Leucocytozoon gametocytes were detected in 17.6% (81/461) of the blood smears from village chickens. The nested polymerase chain reaction (PCR) for targeting Leucocytozoon mitochondrial cytochrome b (cyt b) genes had a 17.6% positive rate. Although the positive rate of nested PCR targeting Plasmodium/Haemoproteus cyt b was 34.3%, the PCR protocol was observed to possibly amplify DNA of a certain species of Leucocytozoon. There were no obvious clinical signs in the infected birds. Statistical analysis of the microscopic detection and PCR detection rates using the age and sex of birds as internal factors revealed that the statistical significances differed according to the study area. The sequencing of 32 PCR pro

Who reads Detection and molecular identification of Leucocytozoon and Plasmodium species from village chickens in different areas of Myanmar?

Author

Shwe Yee Win; Hla Myet Chel; Myint Myint Hmoon; Lat Lat Htun; Saw Bawm; Mar Mar Win; Shiro Murata; Nariaki Nonaka; Ryo Nakao; Ken Katakura

Publisher

Elsevier BV

Published

2020

Language

EN

Field

Medicine (Health Sciences)