Can I read Antagonistic effect of CRP and KdgR in the transcription control of the Erwinia chrysanthemi pectinolysis genes on EtoBox?

Antagonistic effect of CRP and KdgR in the transcription control of the Erwinia chrysanthemi pectinolysis genes by William Nasser; Janine Robert-Baudouy; Sylvie Reverchon is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Antagonistic effect of CRP and KdgR in the transcription control of the Erwinia chrysanthemi pectinolysis genes about?

The main virulence factors of the phytopathogenic bacteria __Erwinia chrysanthemi__ are pectinases that cleave pectin, a major constituent of the plant cell wall. The cyclic AMP receptor protein (CRP) was identified as the main activator of the pectinolysis genes. Gel shift and DNase I footprinting experiments showed that the purified __E. chrysanthemi__ CRP protein binds specifically to the promoter regions of seven pectinolysis genes (__pelB__, __pelC__, __pelD__, __pelE__, __ogl__, __kduI__ and __kdgT__ ) whose expression is positively regulated __in vivo__ by CRP. In contrast, no interaction was observed between CRP and the promoter–operator region of __pelA__, whose expression is negatively regulated __in vivo__ by CRP. Primer extension experiments demonstrated that each of the __pelB__, __pelC__, __pelE__ and __kduI__ genes is expressed from a unique σ^70^ promoter, whereas __ogl__ and __kdgT__ possess three and two functional promoters respectively. The position of the CRP binding site relative to the transcription start site suggests that CRP acts as a primary activator at the __pelB__ (via the CRP binding site 1), __pelC__, __pelE__, __pelD__, __kdgT__ P~1~ and __ogl__P~2~

Who reads Antagonistic effect of CRP and KdgR in the transcription control of the Erwinia chrysanthemi pectinolysis genes?

Author

William Nasser; Janine Robert-Baudouy; Sylvie Reverchon

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

1997

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)