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Molecular characterization of Histoplasma capsulatum isolated from an outbreak in treasure hunters by Bertha Muñoz; María Á Martínez; Gabriel Palma; Amado Ramírez; María G Frías; María R Reyes; María L Taylor; Anjarath L Higuera; Alexander Corcho; María E Manjarrez is a Medicine article available to read on EtoBox.

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## Abstract ## Background In Mexico, primary pulmonary histoplasmosis is the most relevant clinical form of the disease. The geographical distribution of specific strains of__Histoplasma capsulatum__circulating in Mexico has not been fully established. Outbreaks must be reported in order to have current, updated information on this disease, identifying new endemic areas, manner of exposure to the fungi, and molecular characterization of the causative agents. We report a recent outbreak of histoplasmosis in treasure hunters and the molecular characterization of two isolates obtained from these patients. ## Methods Six patients admitted to the National Institute of Respiratory Diseases (INER) in Mexico City presented severe respiratory symptoms suggestive of histoplasmosis. They acquired the infection in the Veracruz (VZ) endemic zone. Diagnosis was made by X-ray and Computed tomography (CT), liver function, immunological techniques, and culture. Identification of__H. capsulatum__isolates was confirmed by using Polymerase chain reaction (PCR) was conducted with a probe from the M antigen, and the isolates were characterized by means of Random amplification of polymorphic DNA (RAPD)-P

Who reads Molecular characterization of Histoplasma capsulatum isolated from an outbreak in treasure hunters?

Author

Bertha Muñoz; María Á Martínez; Gabriel Palma; Amado Ramírez; María G Frías; María R Reyes; María L Taylor; Anjarath L Higuera; Alexander Corcho; María E Manjarrez

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2334)

Published

2010

Language

EN

Field

Medicine (Health Sciences)