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Characterization of a Stretch-activated Potassium Channel in Chondrocytes by Ali Mobasheri; Rebecca Lewis; Judith E.J. Maxwell; Claire Hill; Matthew Womack; Richard Barrett-Jolley is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Characterization of a Stretch-activated Potassium Channel in Chondrocytes about?
## Abstract Chondrocytes possess the capacity to transduce load‐induced mechanical stimuli into electrochemical signals. The aim of this study was to functionally characterize an ion channel activated in response to membrane stretch in isolated primary equine chondrocytes. We used patch‐clamp electrophysiology to functionally characterize this channel and immunohistochemistry to examine its distribution in articular cartilage. In cell‐attached patch experiments, the application of negative pressures to the patch pipette (in the range of 20–200 mmHg) activated ion channel currents in six of seven patches. The mean activated current was 45.9 ± 1.1 pA (__n__ = 4) at a membrane potential of 33 mV (cell surface area approximately 240 μm^2^). The mean slope conductance of the principal single channels resolved within the total stretch‐activated current was 118 ± 19 pS (__n__ = 6), and reversed near the theoretical potassium equilibrium potential, E~K+~, suggesting it was a high‐conductance potassium channel. Activation of these high‐conductance potassium channels was inhibited by extracellular TEA (K~d~ approx. 900 μM) and iberiotoxin (K~d~ approx. 40 nM). This suggests that the current
Who reads Characterization of a Stretch-activated Potassium Channel in Chondrocytes?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ali Mobasheri; Rebecca Lewis; Judith E.J. Maxwell; Claire Hill; Matthew Womack; Richard Barrett-Jolley
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)