Opening book details…
Can I read Hydroethanolic stem bark extracts of Stryphnodendron adstringens impair M1 macrophages and promote M2 polarization on EtoBox?
Hydroethanolic stem bark extracts of Stryphnodendron adstringens impair M1 macrophages and promote M2 polarization by José Tarcísio Giffoni de Carvalho; Juan Sebastian Henao Agudelo; Débora Da Silva Baldivia; Carlos Alexandre Carollo; Denise Brentan Silva; Kely de Picoli Souza; Niels Olsen Saraiva Câmara; Edson Lucas dos Santos is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Hydroethanolic stem bark extracts of Stryphnodendron adstringens impair M1 macrophages and promote M2 polarization about?
## Ethnopharmacological relevance: Stryphnodendron adstringens has been used by indigenous brazilian people to treat wound, infections, inflammation and other conditions. ## Aim of the study: This study aims to investigate the effect of s. adstringens on macrophage polarization. ## Methods: To prepare the hydroethanolic extract of stryphnodendron adstringens (hesa), fresh bark was exposed to maceration, filtered and subsequently lyophilized. the extract hesa were analyzed by lc-dad-ms to identify their constituents. bone marrow cells were obtained from male c57bl/6 mice. then, the cells were polarized into m1 or m2 subsets in the presence or absence of hesa. the membrane expression of tlr2, cd206, ccr7, class ii mhc, and cd86, the intracellular expression of inos and il-6 and the supernatant expression of il-6 were determined by flow cytometry. ## Results: By lc-dad-ms, twenty-four compounds could be detected from hesa and proanthocyanidins, flavan-3-ols, and chromones were identified. no and inos were reduced in the hesa-treated cells. there was a reduction in il6 in hesa-treated cells. the membrane expression of tlr2, cd206, ccr7, cd86, and class ii mhc was reduced in hesa-treate
Who reads Hydroethanolic stem bark extracts of Stryphnodendron adstringens impair M1 macrophages and promote M2 polarization?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- José Tarcísio Giffoni de Carvalho; Juan Sebastian Henao Agudelo; Débora Da Silva Baldivia; Carlos Alexandre Carollo; Denise Brentan Silva; Kely de Picoli Souza; Niels Olsen Saraiva Câmara; Edson Lucas dos Santos
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-8741)
- Published
- 2020
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)