About this document
Iron Determination in Biological Samples by CristhianMoisesCuetoCalderon is a document available to read on EtoBox.
This document describes a colorimetric method for determining iron levels in biological materials using o-phenanthroline. The method involves ashing samples, dissolving the ash, adjusting the pH to 3.5, then adding o-phenanthroline and hydroquinone to form an orange-red complex between ferrous iron and o-phenanthroline. Standards are prepared and absorbance is measured colorimetrically or using a photelometer. The method was tested on foods and shown to accurately recover iron added before and after ashing.
- Author
- CristhianMoisesCuetoCalderon
- Language
- EN