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S-adenosylhomocysteine hydrolase from the archaeon Pyrococcus furiosus: Biochemical characterization and analysis of protein structure by comparative molecular modeling by Marina Porcelli; Maria Angela Moretti; Luigi Concilio; Sabrina Forte; Antonello Merlino; Giuseppe Graziano; Giovanna Cacciapuoti is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is S-adenosylhomocysteine hydrolase from the archaeon Pyrococcus furiosus: Biochemical characterization and analysis of protein structure by comparative molecular modeling about?
## Abstract S‐Adenosylhomocysteine hydrolase (AdoHcyHD) is an ubiquitous enzyme that catalyzes the breakdown of S‐adenosylhomocysteine, a powerful inhibitor of most transmethylation reactions, to adenosine and L‐homocysteine. AdoHcyHD from the hyperthermophilic archaeon __Pyrococcus furiosus__ (PfAdoHcyHD) was cloned, expressed in __Escherichia coli__, and purified. The enzyme is thermoactive with an optimum temperature of 95°C, and thermostable retaining 100% residual activity after 1 h at 90°C and showing an apparent melting temperature of 98°C. The enzyme is a homotetramer of 190 kDa and contains four cysteine residues per subunit. Thiol groups are not involved in the catalytic process whereas disulfide bond(s) could be present since incubation with 0.8 M dithiothreitol reduces enzyme activity. Multiple sequence alignment of hyperthermophilic AdoHcyHD reveals the presence of two cysteine residues in the N‐terminus of the enzyme conserved only in members of __Pyrococcus__ species, and shows that hyperthermophilic AdoHcyHD lack eight C‐terminal residues, thought to be important for structural and functional properties of the eukaryotic enzyme. The homology‐modeled structure of PfA
Who reads S-adenosylhomocysteine hydrolase from the archaeon Pyrococcus furiosus: Biochemical characterization and analysis of protein structure by comparative molecular modeling?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Marina Porcelli; Maria Angela Moretti; Luigi Concilio; Sabrina Forte; Antonello Merlino; Giuseppe Graziano; Giovanna Cacciapuoti
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Liss Inc; Wiley (ISSN 0887-3585)
- Published
- 2005
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)