Can I read Methodological optimization of polyethylenimine (PEI)-based gene delivery to the lungs of mice via aerosol application on EtoBox?

Methodological optimization of polyethylenimine (PEI)-based gene delivery to the lungs of mice via aerosol application by C. Rudolph; A. Ortiz; U. Schillinger; J. Jauernig; C. Plank; J. Rosenecker is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Methodological optimization of polyethylenimine (PEI)-based gene delivery to the lungs of mice via aerosol application about?

## Abstract ## Background Polyethylenimine (PEI) has been successfully used for gene delivery to the lungs of mice via aerosol application using a whole body nebulization device. In this report we optimized the design of such an aerosol device. ## Methods Aerosol devices were constructed as either serial inhalation apparatus or as a whole body nebulization chamber connected to an aerosol spacer placed in a horizontal or vertical position. PEI‐based gene vectors were nebulized using a standard jet nebulizer and luciferase gene expression of various tissues was examined. ## Results Using a whole body aerosol device resulted in luciferase gene expression in the lungs of mice at the same level as compared with a serial inhalation apparatus. Whereas gene expression was enhanced in the presence of 5% CO~2~‐in‐air, anesthesia of mice strongly decreased gene expression even when mice were intubated with an intravascular cannula. Reduction of the median mass aerodynamic diameter (MMAD) of the aerosol from 3.4 to 0.27 μm by interposition of an aerosol spacer increased gene expression significantly 3‐fold. Drying of the aerosol by silica gel additionally increased gene delivery significantly

Who reads Methodological optimization of polyethylenimine (PEI)-based gene delivery to the lungs of mice via aerosol application?

Author

C. Rudolph; A. Ortiz; U. Schillinger; J. Jauernig; C. Plank; J. Rosenecker

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)