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Analysis of an artificially selected GS-NS0 variant with increased resistance to apoptosis by Susan M. Browne; Mohamed Al-Rubeai is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Analysis of an artificially selected GS-NS0 variant with increased resistance to apoptosis about?

## Abstract Heterogeneity in gene expression and phenotypic traits is an inherent feature within the “clonal” cell lines used for biopharmaceutical production. This feature can allow the selection of cell lines with improved phenotypes and adaptation to growth under preferential conditions to improve productivity or provide a platform to study the molecular basis of improved characteristics. A repeated process of extended batch culture of a recombinant antibody producing GS‐NS0 myeloma cell line generated a stable variant cell line displaying increased resistance to both environmental stresses and chemical apoptosis inducers, and resulted in extended culture viability and increased antibody production. An interesting feature of the variant cell line was an altered metabolic state with consumption of lactate as the culture progressed. The variant cell line also showed altered expression of proteins associated with autophagy suggesting a role for this process in extending cell survival in culture. Targeted transcriptomic analysis was carried out on the parental and variant cell lines using a qRT‐PCR array containing a panel of apoptosis‐associated genes to elucidate both the predomin

Who reads Analysis of an artificially selected GS-NS0 variant with increased resistance to apoptosis?

Author

Susan M. Browne; Mohamed Al-Rubeai

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)