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Effects of DMAEMA and 4-methoxyphenol on gingival fibroblast growth, metabolism, and response to interleukin-1 by Carol A. Lapp; George S. Schuster is a Medicine article available to read on EtoBox.

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## Abstract Some components of resins used in restorative dentistry have been shown to alter metabolism in cultured oral epithelial cells. Here we have extended such studies to the underlying supportive tissue, composed of gingival fibroblasts (GF). Primary cultures of human GF were transferred to serum‐free, defined medium and exposed to either 2‐(dimethylamino)ethyl methacrylate (DMAEMA) or 4‐methoxyphenol (MEHQ) for 24–72 h. At a DMAEMA concentration of 6.4 mM, which was well tolerated by epithelial cells, GF numbers, as estimated by crystal violet, and metabolic activity, as indicated by MTT, were reduced at least 60% within 24 h of exposure. Between 1.6 and 6.4 mM, there were dose‐related reductions in cell numbers; however, at lower doses (0.32–0.64 mM), proliferation was stimulated. MEHQ, between 8 and 16 μM, did not stimulate cellular protein production. To examine the capacity of GF to respond to an inflammatory stimulus, interleukin‐6 (IL‐6) production by confluent cells was estimated without or with these compounds. DMAEMA (1.6– 6.4 mM) virtually eliminated the acute IL‐6 response of these cells to an interleukin‐1β challenge; only at 0.32 mM DMAEMA was the response rest

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Author

Carol A. Lapp; George S. Schuster

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Heterocorporation; Wiley (ISSN 0021-9304)

Published

2002

Language

EN

Field

Medicine (Health Sciences)