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Transcriptional interferences at the Hoxa4/Hoxa5 locus: Importance of correct Hhoxa5 expression for the proper specification of the axial skeleton by Josée Aubin; Margot Lemieux; Michel Tremblay; RICHARD R. Behringer; Lucie Jeannotte is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Transcriptional interferences at the Hoxa4/Hoxa5 locus: Importance of correct Hhoxa5 expression for the proper specification of the axial skeleton about?

We have previously described a Hoxa5 mutant mouse line in which specification of axial identity is perturbed and viability is markedly reduced. In the present study, we assay the Hoxa5 mutation in different genetic backgrounds and carry out a complete analysis of skeletal transformations. Although Hoxa5 is expressed over a large domain during embryogenesis, homeotic transformations of the axial skeleton are confined between cervical vertebra C3 and thoracic vertebra T2, which corresponds to the specific expression domain of the major Hoxa5 transcript. Loss of Hoxa5 function also affects the formation of the acromion in the appendicular skeleton. Disruption of the adjacent Hoxa4 gene leads to similar homeotic transformations of the cervicothoracic vertebrae. To discriminate the respective role of each gene, we generated transheterozygous animals carrying inactivated Hoxa4 and Hoxa5 alleles on different chromosomes. Compound heterozygous mutants exhibit homeotic transformations in the cervicothoracic transition region more reminiscent to those observed in Hoxa5 homozygous mutants. Although the Hoxa5 mutation does not significantly affect Hoxa4 expression, the pattern of Hoxa5 express

Who reads Transcriptional interferences at the Hoxa4/Hoxa5 locus: Importance of correct Hhoxa5 expression for the proper specification of the axial skeleton?

Author

Josée Aubin; Margot Lemieux; Michel Tremblay; RICHARD R. Behringer; Lucie Jeannotte

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1058-8388)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)