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Serum stimulation of quiescent human fibroblasts induces the synthesis of tissue factor mRNA followed by the appearance of tissue factor antigen and procoagulant activity by Laura J. Bloem; Lisa Chen; William H. Konigsberg; Ronald Bach is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Expression of the gene for tissue factor, the cell-surface initiator of blood coagulation, was examined by stimulating growth-arrested human fibroblasts with serum and measuring changes in the cellular content of tissue factor mRNA, antigen, and activity. Maximum tissue factor mRNA levels were reached within 1 h following serum induction and slowly declined to basal levels from 24 to 48 h after stimulation. The appearance of the tissue factor mRNA was followed by an increase in tissue factor antigen and activity. The parallel rise in antigenically positive protein and procoagulant activity was first observed about 2 h after serum stimulation with a peak at 12 h followed by a slow decline during the next 36 h. The serum-induced synthesis of the tissue factor mRNA was independent of de novo protein synthesis as demonstrated by the increased tissue factor mRNA levels generated in the presence of cycloheximide. The results of this study suggest that the synthesis of tissue factor in human fibroblasts is regulated principally at the level of transcription. In one strain of fibroblasts the activity/ antigen ratio, during the period of maximum synthesis, was indistinguishable from that of

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Author

Laura J. Bloem; Lisa Chen; William H. Konigsberg; Ronald Bach

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1989

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)