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Heritable CRISPR-Cas9 editing of plant genomes using RNA virus vectors by Mireia Uranga; Verónica Aragonés; José- Antonio Daròs; Fabio Pasin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Heritable CRISPR-Cas9 editing of plant genomes using RNA virus vectors about?

## Summary Viral vectors hold enormous potential for genome editing in plants as transient delivery vehicles of CRISPR-Cas components. Here, we describe a protocol to assemble plant viral vectors for single-guide RNA (sgRNA) delivery. The obtained viral constructs are based on compact T-DNA binary vectors of the pLX series and are delivered into Cas9-expressing plants through agroinoculation. This approach allows rapidly assessing sgRNA design for plant genome targeting, as well as the recovery of progeny with heritable mutations at targeted loci. For complete details on the use and execution of this protocol, please refer to Uranga et al. (2021)^1^ and Aragonés et al. (2022).^2^

Who reads Heritable CRISPR-Cas9 editing of plant genomes using RNA virus vectors?

Author

Mireia Uranga; Verónica Aragonés; José- Antonio Daròs; Fabio Pasin

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)