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Effects of prostaglandin E2 and F2α on cytoplasmic pH in a clonal osteoblast-like cell line, MOB 3–4 by Tomoyuki Kawase; Michiaki Orikasa; Akitoshi Suzuki is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Prostaglandin E, (PGE,, 5 ng/ml to 5 pg/ml) induced a dose-dependent increase in cAMP accumulation, inositol phosphates (IPS) accumulation, and cytoplasmic free Ca2+ ([Ca2+li) in a clonal osteoblast-like cell line, MOB 3-4. In contrast, prostaglandin F,a (PGF a, 5 ng/ml to 5 p,g/ml) stimulated increases in IPS accumulation and ICa';L]i without stimulating an increase in cAMP accumulation. Both PGE, P 0 . 5 pg/ml) and PGF,a (30.5 p,g/ml) increased cytoplasmic pH (pHi) from approximately 7.15 to 7.35 in BCECF-loaded cells. A tumor promotor, phorbol 12-myristate 13-acetate (PMA, 0.1-100 nM) also increased pHi without effect on phosphoinositide hydrolysis. Both PGE,-(5 pg/ml) and PMA-(100 nM) induced cytoplasmic alkalinization was inhibited by removal of extracellular Na+, or by pretreatment of the cells with amiloride (0.5 mM), an inhibitor of Na+/H+ exchange, or H-7 (100 FM), a nonspecific inhibitor of protein kinase C. Thus, MOB 3-4 cells appeared to possess PGE, receptors and PGF,a receptors: the former are coupled to adenylate cyclase and phospholipase C, and the latter are predominantly coupled to phospholipase C. Also the cells appeared to possess an amiloride-sensitive Na+/H+ e

Who reads Effects of prostaglandin E2 and F2α on cytoplasmic pH in a clonal osteoblast-like cell line, MOB 3–4?

Author

Tomoyuki Kawase; Michiaki Orikasa; Akitoshi Suzuki

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1990

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)