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Can I read An Immunoaffinity Column with a Monoclonal Antibody as Ligand for Human Follicle Stimulating Hormone on EtoBox?
An Immunoaffinity Column with a Monoclonal Antibody as Ligand for Human Follicle Stimulating Hormone by Marlene Zandian; Alois Jungbauer is a Chemistry article available to read on EtoBox.
What is An Immunoaffinity Column with a Monoclonal Antibody as Ligand for Human Follicle Stimulating Hormone about?
## Abstract A detailed characterization regarding the chromatographic and biochemical properties of an immunoaffinity column for sample pretreatment using a follicle stimulating hormone (FSH) β‐unit specific mAb has been performed. A maximum binding capacity of approximately 300 μg/mL was determined. A __K__~D~ value of 4.5×10^–8^ M could be obtained in batch isotherm measurements. The dynamic binding capacity at 10% did not change when residence time was at least 6 min. High selectivity could be demonstrated using crude culture supernatant from FSH producing Chinese hamster ovary (CHO) cells. A great stability of the matrix was confirmed by performing 100 consecutive chromatographic runs without loss of capacity.
Who reads An Immunoaffinity Column with a Monoclonal Antibody as Ligand for Human Follicle Stimulating Hormone?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Marlene Zandian; Alois Jungbauer
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 1615-9306)
- Published
- 2009
- Language
- EN
- Field
- Chemistry (Physical Sciences)