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Vertical and Horizontal Transmission of ESBL Plasmid from Escherichia coli O104:H4 by Daniel, Sandra; Goldlust, Kelly; Quebre, Valentin; Shen, Minjia; Lesterlin, Christian; Bouet, Jean-Yves; Yamaichi, Yoshiharu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Vertical and Horizontal Transmission of ESBL Plasmid from Escherichia coli O104:H4 about?

Multidrug resistance (MDR) often results from the acquisition of mobile genetic elements (MGEs) that encode MDR gene(s), such as conjugative plasmids. The spread of MDR plasmids is founded on their ability of horizontal transference, as well as their faithful inheritance in progeny cells. Here, we investigated the genetic factors involved in the prevalence of the IncI conjugative plasmid pESBL, which was isolated from the Escherichia coli O104:H4 outbreak strain in Germany in 2011. Using transposon-insertion sequencing, we identified the pESBL partitioning locus (par). Genetic, biochemical and microscopic approaches allowed pESBL to be characterized as a new member of the Type Ib partitioning system. Inactivation of par caused mis-segregation of pESBL followed by post-segregational killing (PSK), resulting in a great fitness disadvantage but apparent plasmid stability in the population of viable cells. We constructed a variety of pESBL derivatives with different combinations of mutations in par, conjugational transfer (oriT) and pnd toxin-antitoxin (TA) genes. Only the triple mutant exhibited plasmid-free cells in viable cell populations. Time-lapse tracking of plasmid dynamics in

Who reads Vertical and Horizontal Transmission of ESBL Plasmid from Escherichia coli O104:H4?

Author

Daniel, Sandra; Goldlust, Kelly; Quebre, Valentin; Shen, Minjia; Lesterlin, Christian; Bouet, Jean-Yves; Yamaichi, Yoshiharu

Publisher

MDPI AG; Multidisciplinary Digital Publishing Institute (MDPI); Basel: Multidisciplinary Digital Publishing Institute (MDPI), 2010- (ISSN 2073-4425)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)