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Smartphone-based photoelectrochemical immunoassay of prostate-specific antigen based on Co-doped Bi2O2S nanosheets by Qianyun Lin; Zhichao Yu; Liling Lu; Xue Huang; Qiaohua Wei; Dianping Tang is a Engineering article available to read on EtoBox.

What is Smartphone-based photoelectrochemical immunoassay of prostate-specific antigen based on Co-doped Bi2O2S nanosheets about?

Portable and on-site detection of target biomarker is of great significance in early diagnosis of diseases. Herein, we designed a portable smartphone-based PEC immunoassay platform to detect prostate specific antigen (PSA) adopting Co-doped Bi2O2S nanosheets as photoactive materials. The fast photocurrent response under visible light and excellent electrical transport rate invest Co-doped Bi2O2S with the property of being effectively excited even under a weak light source. Therefore, with the incorporation of a carriable flashlight that act as the excitation light source, disposable screen-printed electrodes, a microelectrochemical workstation and a smartphone that served as control center, point-of-care analytical detection of low-abundance small molecule analytes was successfully realized. Specifically, a sandwich-type immunoreaction was performed using alkaline phosphatase labeled secondary antibody as signal indicator. In the presence of PSA, ascorbic acid as generated through a catalytic reaction, resulting in the enhancement of photocurrent intensity. The photocurrent intensity increased linearly with the logarithm of PSA concentrations ranging from 0.2 to 50 ng mL−1 with a d

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Author

Qianyun Lin; Zhichao Yu; Liling Lu; Xue Huang; Qiaohua Wei; Dianping Tang

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Engineering (Physical Sciences)