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Hypermethylated RASSF1 and SLC5A8 promoters alongside BRAF^V600E^ mutation as biomarkers for papillary thyroid carcinoma by Fatemeh Khatami; Bagher Larijani; Ramin Heshmat; Shirzad Nasiri; Mohammad Haddadi‐Aghdam; Ladan Teimoori‐Toolabi; Seyed M. Tavangar is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Hypermethylated RASSF1 and SLC5A8 promoters alongside BRAF^V600E^ mutation as biomarkers for papillary thyroid carcinoma about?
## Abstract Circulating cell‐free DNA (cfDNA) has been considered as a diagnostic source to track genetic and epigenetic alterations in cancer. We aimed to study mutation in addition to the methylation status in the promoter regions of RASSF1 and SLC5A8 genes in tissues and circulating free DNA samples of patients affected with papillary thyroid carcinoma (PTC) and thyroid nodules as controls. BRAF^V600E^ mutation was studied by ARMS‐scorpion real‐time polymerase chain reaction method in 57 PTC and 45 thyroid nodule cases. Methylation status of RASSF1 and SLC5A8 promoter regions was analyzed by methylation‐specific high‐resolution melting curve analysis. BRAF^V600E^ mutation was found in 39 (68.4%) out of 57 PTC tissue samples, while in 33 (49.1%) cases of cfDNA, this mutation was detected. The frequency of BRAF^V600E^ mutation in cfDNA was significantly different between metastatic and nonmetastatic PTC cases (22 of 33 PTC cases vs. 5 of 34 thyroid nodule samples). Methylation levels of three promoter regions of SLC5A8 and proximal promoter region of RASSF1 was significantly different between PTC and thyroid nodule cases in both cfDNA and tissue DNA. In addition, the methylation s
Who reads Hypermethylated RASSF1 and SLC5A8 promoters alongside BRAF^V600E^ mutation as biomarkers for papillary thyroid carcinoma?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Fatemeh Khatami; Bagher Larijani; Ramin Heshmat; Shirzad Nasiri; Mohammad Haddadi‐Aghdam; Ladan Teimoori‐Toolabi; Seyed M. Tavangar
- Publisher
- Wiley
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)