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Thermodynamic Characterization of the Adsorption of Selected Chiral Compounds on Immobilized Amyloglucosidase in Liquid Chromatography by Gustaf Götmar; Jörgen Samuelsson; Anders Karlsson; Torgny Fornstedt is a Chemistry article available to read on EtoBox.

What is Thermodynamic Characterization of the Adsorption of Selected Chiral Compounds on Immobilized Amyloglucosidase in Liquid Chromatography about?

Immobilized amyloglucosidase was used as a chiral stationary phase (CSP). First, the retention and enantioselectivity of several model chiral amines and acids were investigated. We found that this CSP was unable to separate the enantiomers of acids, though all selected amines could be resolved. The adsorption of (R)- and (S)-propranolol and its influence on column temperature and 2-propanol content in the eluent were then studied in detail, using a three-step methodology. The adsorption was first evaluated using Scatchard plots; thereafter, the adsorption was characterized in detail by calculating the adsorption energy distribution. With this model-independent information, a better judgment could be made of the possible adsorption models selected in the last step, the model fitting to the data. In the case examined, the bi-Langmuir model (containing nonselective and enantioselective sites) describes the system well. The retention of (R)- and (S)-propranolol at low temperatures increases with the content of 2-propanol in the eluent, due to the increased saturation capacity of the enantioselective sites. The retention is an enthalpy-driven process at both types of sites, whereas the

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Author

Gustaf Götmar; Jörgen Samuelsson; Anders Karlsson; Torgny Fornstedt

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2007

Language

EN

Field

Chemistry (Physical Sciences)