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Effect of Cyclic Axial Stretch of Rat Arteries on Endothelial Cytoskeletal Morphology and Vascular Reactivity by Pieter Sipkema; Peter J.W van der Linden; Nico Westerhof; Frank C.P Yin is a Medicine article available to read on EtoBox.

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Pulsatile fluid shear stress and circumferential stretch are responsible for the axial alignment of vascular endothelial cells and their actin stress fibers in vivo. We studied the effect of cyclic alterations in axial stretch independent of flow on endothelial cytoskeletal organization in intact arteries and determined if functional alterations accompanied morphologic alterations. Rat renal arteries were axially stretched (20%, 0.5 Hz) around their in vivo lengths, for up to 4h. Actin stress fibers were examined by immunofluorescent staining. We found that cyclic axial stretching of intact vessels under normal transmural pressure in the absence of shear stress induces within a few hours realignment of endothelial actin stress fibers toward the circumferential direction. Concomitant with this morphologic alteration, the sensitivity (log(EC(50))) to the endothelium-dependent vasodilator (acetylcholine) was significantly decreased in the stretched vessels (after stretching -5.15+/-0.79 and before stretching -6.71+/-0.78, resp.), while there was no difference in sodium nitroprusside (SNP) sensitivity. There was no difference in sensitivity to both acetylcholine and SNP in time control

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Author

Pieter Sipkema; Peter J.W van der Linden; Nico Westerhof; Frank C.P Yin

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0021-9290)

Published

2003

Language

EN

Field

Medicine (Health Sciences)