Opening book details…
Can I read Sclerostin Ablation Prevents Aortic Valve Stenosis in Mice on EtoBox?
Sclerostin Ablation Prevents Aortic Valve Stenosis in Mice by J. Ethan Joll; Lance A. Riley; Matthew R. Bersi; Jeffry S. Nyman; W. David Merryman is a Medicine article available to read on EtoBox.
What is Sclerostin Ablation Prevents Aortic Valve Stenosis in Mice about?
The objective of this study was to test the hypothesis that targeting sclerostin would accelerate the progression of aortic valve stenosis. Sclerostin (mouse gene, ) is a secreted glycoprotein that acts as a potent regulator of bone remodeling. Antibody therapy targeting sclerostin is approved for osteoporosis but results from a clinical trial showed multiple off-target cardiovascular effects. Wild-type (WT, ) and -gene knockout-expression (Null, ) mice were generated and maintained to 12 mo of age on a high-cholesterol diet to induce aortic valve stenosis. Mice were examined by echocardiography, histology, and RNAseq. Immortalized valve interstitial cells were developed from each genotype for in vitro studies. Null mice developed a bone overgrowth phenotype, similar to patients with sclerosteosis. Surprisingly, however, WT mice developed hemodynamic signs of aortic valve stenosis, whereas Null mice were unchanged. WT mice had thicker aortic valve leaflets and higher amounts of α-smooth muscle actin, a marker myofibroblast activation and dystrophic calcification, with very little evidence of Runx2 expression, a marker of osteogenic calcification. RNAseq analysis of aortic roots ind
Who reads Sclerostin Ablation Prevents Aortic Valve Stenosis in Mice?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- J. Ethan Joll; Lance A. Riley; Matthew R. Bersi; Jeffry S. Nyman; W. David Merryman
- Publisher
- American Physiological Society
- Published
- 2022
- Language
- EN
- Field
- Medicine (Health Sciences)