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Modulation of the Uterine Response to Infectious Bacteria in Postpartum Ewes by RICHARD C. SEALS; MEGHAN C. WULSTER-RADCLIFFE; GREGORY S. LEWIS is a scholarly article available to read on EtoBox.

What is Modulation of the Uterine Response to Infectious Bacteria in Postpartum Ewes about?

## Problem: Exogenous progesterone and prostaglandin e2 (pge2) can downregulate uterine immune functions and render the uterus susceptible to bacterial infection. ## Method of study: Ewes were sham-ovariectomized (sham) or ovariectomized (ovex) 9 days after parturition (day 0), and their uteri were inoculated with arcanobacterium pyogenes and escherichia coli on day 15. vena caval blood was collected on day 14 and days 16-19, and uteri were collected on day 20. ewes began receiving either canola oil (oil) or progesterone in oil (prog) on day 10. lymphocytes from each blood sample were assigned to a 2 x 2 factorial array of in vitro treatments; 10(-7) m pge2 and 10(-7) m indomethacin (indo) were main effects. [3h]thymidine incorporation (expressed in picomoles) was used to quantify proliferation. ## Results: Progesterone was greater (p = 0.001) in prog than in oil ewes (3.6 versus 0.7 ng/ml), and only prog ewes developed infections. lymphocyte proliferation was least (p = 0.02) in prog-ovex ewes (4.1 versus 5.4, 5.7, and 5.8 pmol for oil-sham, prog-sham, and oil-ovex, respectively). concanavalin a (con-a)-stimulated proliferation was less (p < 0.01) for pge2- and pge2 + indo-treated

Author

RICHARD C. SEALS; MEGHAN C. WULSTER-RADCLIFFE; GREGORY S. LEWIS

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 8755-8920)

Published

2002

Language

EN