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Software-based quantitation of bioassays on CD by Manu Pallapa; Lily M.L. Ou; M. (Ash) Parameswaran; Hua-Zhong Yu is a Engineering article available to read on EtoBox.

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## a b s t r a c t Compact discs (CDs) can be used as rapid, low-cost, high-capacity screening platforms for running bioassays with no modification of the detection hardware (conventional standard optical drive). We describe herein a new protocol to read and quantitate biotin-streptavidin binding assays with a standard optical drive by using a CD-data analysis software (IsoBuster), which identifies erroneous sectors by locating the exact error position bit-by-bit and allows various data formats to be used. The numbers of erroneous sectors increase as a function of the streptavidin concentration in the tested solutions. High spatial accuracy and detection sensitivity (0.3 g/mL) were achieved.

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Author

Manu Pallapa; Lily M.L. Ou; M. (Ash) Parameswaran; Hua-Zhong Yu

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0925-4005)

Published

2010

Language

EN

Field

Engineering (Physical Sciences)