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An in Vitro Test for Measuring Cytotoxicity of Mercuric Chloride to Human Kidney Epithelial Cells by Specific Enzyme Release by D. Merlet; M. Mellado; J. P. Merlet; J. Cambar is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is An in Vitro Test for Measuring Cytotoxicity of Mercuric Chloride to Human Kidney Epithelial Cells by Specific Enzyme Release about?

Mercuric compound toxicity is well documented in animals and man for practically all organs. The recent development of cell culture techniques appeared as a novel fruitful tool in toxicology, especially in renal toxicology. Heavy metal induced renal cell alterations can be evaluated by membrane permeability damages. The present study evaluates mercuric chloride nephrotoxic effect in human kidney epithelial cells by measuring the release of two specific nephrotoxicity marker enzymes, Gamma Glutamyl Transferase (GGT) and Alkaline Phosphatase (ALP) in the culture medium. Cultured kidney epithelial cells were exposed to different HgC12 concentrations (5, 10, 20, and 50 lxg). Cultures were examined after 6 and 24 hours exposure. A good correlation between mercury dose and toxic effect, and exposure time and toxic effect was found. Enzymes were significantly released into the culture medium for 5 gg and 10 gg HgC12/ml after 6 hours exposure; and after 24 hours exposure, enzymes were released for 5 gg/ml only. It appears that the specific tubular enzyme release in the culture medium is a good in vitro test for quantification of specific tubular damage.

Who reads An in Vitro Test for Measuring Cytotoxicity of Mercuric Chloride to Human Kidney Epithelial Cells by Specific Enzyme Release?

Author

D. Merlet; M. Mellado; J. P. Merlet; J. Cambar

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC (ISSN 0920-9069)

Published

1988

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)