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High-performance Thin-layer Chromatographic Analysis of Dialysable Materials in Allergen Extracts by R. Wahl*; H.J. Maasch; W. Geissler is a Chemistry article available to read on EtoBox.
What is High-performance Thin-layer Chromatographic Analysis of Dialysable Materials in Allergen Extracts about?
Allergen extracts are used in human medicine for diagnosis and therapy of IgE-mediated allergic diseases. In the crude form they are complex mixtures of proteins, glycoproteins, peptides, carbohydrates and components of low molecular weight. Low-molecular-weight components, e.g. free histamine and substances that may liberate histamine or other mediators in humans, are thought to cause nonspecific side-reactions in patients. It has therefore been recommended that these substances should be removed from the extracts by appropriate methods'. An accepted procedure for the partial purification of allergen extracts is dialysis, which removes components of molecular weight below a certain cut-off limit to a high degree. However, it is necessary to verify that the allergenic activity of an extract is not affected. This paper reports the analysis of non-dialysed and dialysed allergen extracts of the pollens of birch, six-grass mixture and English plantain and of the mould Alternariu tenuis by high-performance thin-layer chromatography (HFTLC). The dyeing reagents used were ninhydrin, iodine and molybdatophosphoric acid. The sensitivities of the dyeing reagents were tested with diluted solu
Who reads High-performance Thin-layer Chromatographic Analysis of Dialysable Materials in Allergen Extracts?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- R. Wahl*; H.J. Maasch; W. Geissler
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 1985
- Language
- EN
- Field
- Chemistry (Physical Sciences)