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Hepcidin Expression in Human Airway Epithelial Cells Is Regulated by Interferon-γ by Marie D Frazier; Lisa B Mamo; Andrew J Ghio; Jennifer L Turi is a scholarly article available to read on EtoBox.

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## Background Hepcidin serves as a major regulator of systemic iron metabolism and immune function. Airway epithelial cells have an extensive interface with the environment, and so must be able to respond locally to the presence of particulates, infection, and inflammation. Therefore, we hypothesized that hepcidin is expressed in airway epithelial cells and is regulated by early phase cytokines. ## Methods Primary, differentiated human bronchial epithelial (NHBE) cells were used to assess hepcidin gene expression in response to IFN-γ, TNF-α, IL-1β, and IL-6, as well as to LPS + CD14. The role of the Janus Kinase-signal transducer and activator of transcription (JAK-STAT) pathway in IFN-γ-mediated hepcidin production was assessed by measuring JAK2 phophorylation and STAT1 nuclear translocation. Inductively coupled plasma mass spectroscopy (ICP-MS) was used to determine whether hepcidin altered iron transport in either NHBE cells or primary alveolar macrophages. ## Results We demonstrate that differentiated human airway epithelial cells express hepcidin mRNA and that its expression is augmented in response to IFN-γ via activation of STAT1. However, while IFN-γ induced hepcidin gene e

Author

Marie D Frazier; Lisa B Mamo; Andrew J Ghio; Jennifer L Turi

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: Current Science Ltd, 2000-; [London]: BioMed Central; Springer Science and Business Media LLC (ISSN 1465-9921)

Published

2011

Language

EN