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Suppression of oncogenic NRAS by RNA interference induces apoptosis of human melanoma cells by Malihe Eskandarpour; Shahryar Kiaii; Chaoyong Zhu; Juan Castro; Andrew J. Sakko; Johan Hansson is a Medicine article available to read on EtoBox.
What is Suppression of oncogenic NRAS by RNA interference induces apoptosis of human melanoma cells about?
## Abstract The majority of human melanomas harbor activating mutations in either the __BRAF__ or __NRAS__ gene. To date, the role of oncogenic __NRAS__ in melanoma remains poorly defined and no current therapies are directed at specifically suppressing oncogenic __NRAS__ in human melanoma tumors. The aim of our study, therefore, was to investigate the effects of suppressing oncogenic __NRAS__ in human melanoma cell lines __in vitro__. Using both small interfering RNA‐ and plasmid based‐RNA interference techniques, oncogenic __NRAS__ was specifically suppressed in 2 human melanoma cell lines, 224 and BL, which harbor a codon 61 CAA (glutamine) to CGA (arginine) __NRAS__ mutation. Suppression of oncogenic __NRAS__ in these cell lines resulted in increased apoptosis. Furthermore, in 224 cells we demonstrated decreased phosphorylation of extracellular signal‐regulated kinase (ERK) and Akt, and reduced expression of NF‐κB and cyclin D1 in the N‐Ras signaling pathway. In contrast, RNA interference directed at wild‐type (WT) __NRAS__ had no significant effect on apoptosis of 224 cells or 2 human melanoma cell lines (A375 and 397) containing WT __NRAS__ but a codon 600 GTG (valine) to GAG
Who reads Suppression of oncogenic NRAS by RNA interference induces apoptosis of human melanoma cells?
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- Author
- Malihe Eskandarpour; Shahryar Kiaii; Chaoyong Zhu; Juan Castro; Andrew J. Sakko; Johan Hansson
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2005
- Language
- EN
- Field
- Medicine (Health Sciences)