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Can I read MBD4 cooperates with DNMT1 to mediate methyl-DNA repression and protects mammalian cells from oxidative stress on EtoBox?
MBD4 cooperates with DNMT1 to mediate methyl-DNA repression and protects mammalian cells from oxidative stress by Sophie Laget; Benoit Miotto; Hang Gyeong Chin; Pierre-Olivier Estève; Richard J Roberts; Sriharsa Pradhan; Pierre-Antoine Defossez is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is MBD4 cooperates with DNMT1 to mediate methyl-DNA repression and protects mammalian cells from oxidative stress about?
Oxidative stress induces genome-wide remodeling of the chromatin structure. In this study, we identify Methyl-CpG Binding Protein 4 (MBD4), a multifunctional enzyme involved in DNA demethylation, base excision repair, and gene expression regulation, as an essential factor in response to oxidative stress. We provide evidence that MBD4 is upregulated at the protein level upon oxidative stress, and that MBD4 is essential for cell survival following oxidative stress. In these cells, MBD4 and DNMT1 are recruited at sites of oxidation-induced DNA damage, where we speculate they participate in DNA repair. MBD4 and DNMT1 also share genomic targets in unstressed cells. Using genome-wide analysis of MBD4 binding sites, we identified new targets potentially co-regulated by MBD4 and DNA methylation. We identified two new binding sites for MBD4 and DNMT1 at methylated CpG islands of __CDKN1A/p21__ and __MSH4,__ where they synergistically mediate transcriptional repression. Our study provides evidence that the interaction between DNMT1 and MBD4 is involved in controlling gene expression and responding to oxidative stress.
Who reads MBD4 cooperates with DNMT1 to mediate methyl-DNA repression and protects mammalian cells from oxidative stress?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Sophie Laget; Benoit Miotto; Hang Gyeong Chin; Pierre-Olivier Estève; Richard J Roberts; Sriharsa Pradhan; Pierre-Antoine Defossez
- Publisher
- Informa UK Limited
- Published
- 2014
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)