Can I read Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus on EtoBox?
Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus by Kian-Chung Lee; Daina Lim; Sek-Man Wong; Terje Dokland is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus about?
Hibiscus chlorotic ringspot virus (HCRSV), a Carmovirus, occurs worldwide and induces chlorotic ringspots on leaves, stunting and flower distortion in Hibiscus species, including kenaf. The HCRSV capsid has T = 3 icosahedral symmetry and contains 180 copies of the coat protein. A virus yield of 48-70 mg per 100 g of infected kenaf leaves was achieved with an improved purification scheme involving sucrose-cushion and sucrose density-gradient centrifugation. The virus was crystallized using PEG 8000 and 2,3-butanediol as co-precipitants. The crystals belonged to the cubic space group P23, with unit-cell parameter a = 392 A, and diffracted X-rays to at least 4.5 A resolution.
Who reads Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Kian-Chung Lee; Daina Lim; Sek-Man Wong; Terje Dokland
- Publisher
- International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)
- Published
- 2003
- Language
- EN
- ISBN
- 9780907444909
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)