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Direct and Rapid Measurement of Hydrogen Peroxide in Human Blood Using a Microfluidic Device by Gaikwad, R. (author);Thangaraj, P. R. (author);Sen, A. K. (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The levels of hydrogen peroxide ($${\mathrm{H}}\_{2}{\mathrm{O}}\_{2}$$ H 2 O 2 ) in human blood is of great relevance as it has emerged as an important signalling molecule in a variety of disease states. Fast and reliable measurement of $${\mathrm{H}}\_{2}{\mathrm{O}}\_{2}$$ H 2 O 2 levels in the blood, however, continues to remain a challenge. Herein we report an automated method employing a microfluidic device for direct and rapid measurement of $${\mathrm{H}}\_{2}{\mathrm{O}}\_{2}$$ H 2 O 2 in human blood based on laser-induced fluorescence measurement. Our study delineates the critical factors that affect measurement accuracy—we found blood cells and soluble proteins significantly alter the native $${\mathrm{H}}\_{2}{\mathrm{O}}\_{2}$$ H 2 O 2 levels in the time interval between sample withdrawal and detection. We show that separation of blood cells and subsequent dilution of the plasma with a buffer at a ratio of 1:6 inhibits the above effect, leading to reliable measurements. We demonstrate rapid measurement of $${\mathrm{H}}\_{2}{\mathrm{O}}\_{2}$$ H 2 O 2 in plasma in the concentration range of 0–49 μM, offering a limit of detection of 0.05 μM, a sensitivity of

Who reads Direct and Rapid Measurement of Hydrogen Peroxide in Human Blood Using a Microfluidic Device?

Author

Gaikwad, R. (author);Thangaraj, P. R. (author);Sen, A. K. (author)

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)