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Can I read An Experimental Study of Amino Acid Degradation Under Open Flask Hydrolytic Conditions on EtoBox?
An Experimental Study of Amino Acid Degradation Under Open Flask Hydrolytic Conditions by Angelo Mondino; Gianni Bongiovanni is a Chemistry article available to read on EtoBox.
What is An Experimental Study of Amino Acid Degradation Under Open Flask Hydrolytic Conditions about?
The authors suggest that the open flask system for acid hydrolysis of proteins could also be used to advantage when it is necessary to submit small quantities of pure and isolated proteins to acid hydrolysis. This method is simpler and offers a higher repeatibility, regarding the removal of dissolved oxygen, as against the sealed vial system. An experimental study of the behaviour of an artificial protein amino acid mixture, under standard open flask hydrolytic conditions, in constant boiling hydrochloric acid and in an oxygen-free atmosphere, is reported. The interaction of tryptophan and cystine under these conditions with formation of cysteine was investigated. It was found that this amino acid can severely interfere with the chromatographic separation of proline, when sodium buffers are employed in the elution of resin columns. The separation of these two amino acids has been achieved by employing a method which uses lithium buffers and which has been previously described by one of the authors for the separation of amino acids and related compounds in physiological fluids.
Who reads An Experimental Study of Amino Acid Degradation Under Open Flask Hydrolytic Conditions?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Angelo Mondino; Gianni Bongiovanni
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 1970
- Language
- EN
- Field
- Chemistry (Physical Sciences)