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Effect of gamma interferon on HLA class-I and -II transcription and protein expression in human breast adenocarcinoma cell lines by Nabila Jabrane-Ferrat; Annick Faille; Pascale Loiseau; Odette Poirier; Dominique Charron; Fabien Calvo is a Medicine article available to read on EtoBox.

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## Abstract The spontaneous expression of HLA class‐I and class‐II molecules in 5 human breast carcinoma cell lines, MCF‐7, T47D, ZR75‐I, HSL‐53, MDA‐MB 231, and their modulation during IFN‐~γ~ treatment, are reported. The expression of cell‐surface determinants was examined by indirect immunofluorescence using monoclonal antibodies (MAbs) specific for HLA class‐I and class‐II (DR, DQ and DP) antigens. The biosynthesis and maturation of these molecules were analyzed by 2‐dimensional gel electrophoresis analysis (2D‐PAGE) of class I, DRα, β and invariant immunoprecipitates. Transcription was analyzed by Northern blot hybridization with HLA class‐I and ‐II cDNA‐specific probes. In all cell lines, more than 80% of cells expressed HLA class‐I antigens at their surface. 2D‐PAGE and mRNA studies showed a variable basal level of HLA class‐I biosynthesis and transcription with a constant increase after 1,000 U/ml IFN‐~γ~ treatment. HLA class‐II determinants were totally absent from the surface of MCF‐7, MDA MB231, ZR75‐I and T47D but they were detected in a small subpopulation of HSL‐53 cells (DR 6%, DQ 6%, DP 20%). Spontaneous biosynthesis of HLA‐DR molecules in immunoprecipitates analyze

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Author

Nabila Jabrane-Ferrat; Annick Faille; Pascale Loiseau; Odette Poirier; Dominique Charron; Fabien Calvo

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1990

Language

EN

Field

Medicine (Health Sciences)