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Can I read An Original Approach to Determining Traces of Tetracycline Antibiotics in Milk and Eggs by Solid-phase Extraction and Liquid Chromatography/mass Spectrometry on EtoBox?
An Original Approach to Determining Traces of Tetracycline Antibiotics in Milk and Eggs by Solid-phase Extraction and Liquid Chromatography/mass Spectrometry by Federica Bruno; Roberta Curini; Antonio Di Corcia; Manuela Nazzari; Marco Pallagrosi is a Chemistry article available to read on EtoBox.
What is An Original Approach to Determining Traces of Tetracycline Antibiotics in Milk and Eggs by Solid-phase Extraction and Liquid Chromatography/mass Spectrometry about?
An original and highly specific method able to identify and quantify traces of five tetracycline antibiotics (TCAs) in milk and eggs is presented. This method uses a single solid-phase extraction (SPE) cartridge for simultaneous extraction and purification of TCAs in the above matrices. After diluting 5 mL of intact whole milk or 2 g egg samples with Na(2)EDTA-containing water, samples are passed through a 0.5-g Carbograph 4 extraction cartridge. After analyte elution from the SPE cartridge, an aliquot of the final extract is injected into a liquid chromatography/mass spectrometry (LC/MS) instrument equipped with an electrospray ion source and a single quadrupole. MS data acquisition is performed in the positive-ion mode and by a time-scheduled multiple-ion selected ion-monitoring program. With methanol as organic modifier, the in-source collision-induced dissociation (CID) process generated fragment ions able to pick up one methanol molecule. In several cases, these methanol-adduct fragment ions have m/z values higher than those of the protonated molecules. This event is rarely encountered in MS, thus making the analysis of TCAs by this method extremely specific. Compared with a c
Who reads An Original Approach to Determining Traces of Tetracycline Antibiotics in Milk and Eggs by Solid-phase Extraction and Liquid Chromatography/mass Spectrometry?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Federica Bruno; Roberta Curini; Antonio Di Corcia; Manuela Nazzari; Marco Pallagrosi
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)
- Published
- 2002
- Language
- EN
- Field
- Chemistry (Physical Sciences)